ABSTRACT
Objective The multiple polymerase chain reaction(PCR) technique was adopted to detect AZF microdeletion of Y chromosome in 120 patients with idiopathic oligozoospermia and azoospermia and contemporaneous 60 cases of normal semen rou‐tine examination in the outpatient department .At the same time the peripheral blood chromosome karyotype analysis was performed on these 120 patients .Methods Multiple PCR (Polymerase chain reaction) technique were used to analyze 15 locus of the azoosper‐mia factor (AZF) in the 120 patients with idiopathic azoospermia and oligozoospermia and 60 mormal cases .using the Karyotype a‐nalysis technique to analyze karyotype of the 120 male infertile patients ,at the same time .Results Among 120 cases of male infer‐tility ,no obvious abnormal karyotype was found .23 cases were detected AZFc deletions with the detection rate of 19 .2% ,no mi‐crodeletions at other loci were detected .Conclusion AZFc is the major candidate gene for AZF gene screening in male infertility pa‐tients from this area .The Y chromosome microdeletion is the important cause leading to male infertility .
ABSTRACT
The laboratory animal science, a comprehensive discipline researching on experimental animal and animal experimentation, is the life sciences research foundation and the support condition. It has very vital significance to sudy the related discipline especially the life sciences. This article discusses the construction of the status of Laboratory Animal Science course combined with the development of laboratory animal science and contacted with the characteristics of medical colleges and related disciplines.
ABSTRACT
Objective To study the clinical significance of HBV preS1-Ag,HBV-DNA and HBeAg in main groups of HBV infection.Methods HBV preS1-Ag and hepatitis B serum markers individually were detected by ELISA,and HBV-DNA by fluorescence quantitative PCR, then the detection results of the 308 cases were analysed.Results The serum markers of the 308 cases infected by HBV presented four modes which were "HBsAg,HBeAg,HBcAb","HBsAg,HBeAb,HBcAb","HBsAg,HBeAg","HBsAg,HBcAb".The total positive rate of preS1-Ag was 62.34%(192/308),and HBV-DNA was 78.90%(243/308).Compared HBeAg(+) groups with HBeAg(-)/anti-HBe(+) groups, the corresponding positive rates of preS1-Ag were 71.65%(139/194) and 46.49%(53/114),respectively.The difference of the two groups was significant (?~2=18.30;P